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Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.

Western blot analysis using beta-Actin mouse mAb against Rat serum(1),Rabbit serum(2),PC-12(3),Raw264.7(4),NIH/3T3(5),NRK(6),C2C12(7),C6(8),L1210(9),F9(10),CHO3D10(11),Hela(12),Jurkat(13) cell lysate.

Immunoprecipitation using beta-Actin mouse mAb(dilution: 1/250) against HL-60,C2C12,NRK,RSC,Hela,Cos7 cell lysate.

Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).

Mouse Monoclonal Antibody to beta-Actin

貨號：
20270
別名：
PS1TP5BP1; ACTB
產品上架時間：
2024-03-13
應用：
WB,IP,IF,FCM
反應種屬：
Human,Mouse,Hamster,Rat,Monkey,Dog
抗體類型：
Loading Control Antibody
Swissprot：
P60709
規格：

100μl
數量：

-

+
說明書：

目錄價￥1320

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Mouse Monoclonal Antibody to beta-Actin

Description
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Description

Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

References
1. Proteomics. 2005 Oct;5(15):3876-84. 2. PLoS Med. 2005 Oct;2(10):e263. 3. Mol Biol Cell. 2005 Nov;16(11):5055-60 4. Nature. 2005 Oct 20;437(7062):1173-8.

Specification
Aliases	PS1TP5BP1; ACTB
Entrez GeneID	60
Swissprot	P60709
clone	8H10D10
WB Predicted band size	42kDa
Host/Isotype	Mouse IgG2b
Antibody Type	Loading Control Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Hamster,Rat,Monkey,Dog
Immunogen	Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Formulation	Purified antibody in PBS with 0.05% sodium azide.